RAW MATERIALS IDENTIFICATION AND MANUFACTURED PRODUCTS AUTHENTICATION TECHNOLOGIES
Ключевые слова:
grapes, wine, variety, Vitis vinifera L, sample preparation, extraction, DNA, identification, authentication, gene, UFGT, SNP, PCR, sequencing.Аннотация
DNA technologies for the raw grapes identification and the authentication of wine products made
from them are popular scientific and practical direction with the prospect of introducing developments into the
quality management system. The molecular genetic approaches development to the technical grape varieties’ genetic
identification and wines DNA authentication produced from them was the aim of this work. Sample preparation of
experimental wines was carried out by exposure in a mixture with a precipitant and a co-precipitant, followed by
wine debris precipitation. The nucleic acids extraction from wine debris was carried out with the same commercial
kit as from the pulp of a mature grape fruit, but with an additional lysis solution modification. The obtained results
indicated the selected conditions effectiveness for sample preparation, DNA isolation and PCR for amplification of
the analyzed Vitis vinifera L. UFGT-gene loci. The described strategy for the raw materials geno-identification and
the manufactured products authentication is based on the polymorphic positions of diagnostically significant UFGTgene-associated groups analysis, interpreted by sequencing the corresponding PCR product.
